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KMID : 0385219990090010030
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Korean Journal of Gerontology
1999 Volume.9 No. 1 p.30 ~ p.37
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Cytotoxicity of Methyl Gallate KB Cells
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Abstract
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In the present study, we have evaluated cytotoxic effects of methyl gallate(MG) in human oral epitheloid carcinoma cells (KB cells). Antineoplastic activity was measured by colorimetric assays using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-dipohenyl-2H-tetrazolium bromide (MTT) and sulforhodamine protein B (SRB). The light microscopic study showed morphological changes, Ag-NOR (argyrophylic nucleolar organizer region) number and PAS positive reaction of the treated cells. These results were obtained as follows: MTT and SRB quantities were significantly decreased in cultured KB cells treated with 65.55 mM and 168.81 mM. The number of Ag-NORs were significantly decreased in KB cells treated with MG. PAS reaction of KB cells treated with 50 mM and 100 mM was negative. These results suggest that MG may be plays some role on a cytotoxic activity in KB cell.
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KEYWORD
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Methyl gallate, MTT assay, Sulforhordamine protein B assay, Argyrophylic nucleolar organizer region, Periodic acid - schiff reaction
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